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three-fragment multisite gateway system  (Thermo Fisher)


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    Structured Review

    Thermo Fisher three-fragment multisite gateway system
    Three Fragment Multisite Gateway System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/three-fragment+multisite+gateway+system/three+fragment+multisite+gateway+system/pm40224011-344-10-15
    Average 90 stars, based on 1 article reviews
    three-fragment multisite gateway system - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Expressing:

    Article Title: The exocytosis regulator complexin controls spontaneous synaptic vesicle release in a CAPS-dependent manner at C. elegans excitatory synapses.
    Article Snippet: All expression plasmids were constructed with a three-fragment multisite gateway system (Invitrogen, Thermo Fisher Scientific, Waltham, Massachusetts, United States of America).

    Article Title: Molecular and circuit mechanisms underlying avoidance of rapid cooling stimuli in C. elegans.
    Article Snippet: Three-fragment multisite gateway system (Invitrogen, Thermo Fisher Scientific, USA) and In-fusion (Clontech Laboratories, Inc., USA) methods were used to generate most plasmids as previously described34,73,74.

    Article Title: Sensory plasticity caused by up-down regulation encodes the information of short-term learning and memory.
    Article Snippet: All expression plasmids used in this study were constructed by Three-Fragment Multisite gateway system (InvitrogenTM, Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Tissue-Specific Transcription Footprinting Using RNA PoI DamID (RAPID) in Caenorhabditis elegans
    Article Snippet: Reporter plasmids were assembled using the three-fragment MultiSite Gateway system (Thermo Fisher Scientific).

    Article Title: Molecular and circuit mechanisms underlying avoidance of rapid cooling stimuli in C. elegans
    Article Snippet: Three-fragment multisite gateway system (Invitrogen, Thermo Fisher Scientific, USA) and In-fusion (Clontech Laboratories, Inc., USA) methods were used to generate most plasmids as previously described , , .

    Article Title: GCY-20 signaling controls suppression of Caenorhabditis elegans egg laying by moderate cold.
    Article Snippet: All expression constructs were generated with a Three-Fragment Multisite Gateway system (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Dissecting Molecular and Circuit Mechanisms for Inhibition and Delayed Response of ASI Neurons during Nociceptive Stimulus.
    Article Snippet: Briefly, three entry clones containing three PCR products (Promoter, gene of interest, sl2::GFP, or 30UTR, in name of slot1, slot2 and slot3, respectively) were recombined into the pDESTTM R4-R3 Vector II or custom-modified destination vectors using LR recombination reactions to generate the expression plasmids.

    Construct:

    Article Title: The exocytosis regulator complexin controls spontaneous synaptic vesicle release in a CAPS-dependent manner at C. elegans excitatory synapses.
    Article Snippet: All expression plasmids were constructed with a three-fragment multisite gateway system (Invitrogen, Thermo Fisher Scientific, Waltham, Massachusetts, United States of America).

    Article Title: Molecular and circuit mechanisms underlying avoidance of rapid cooling stimuli in C. elegans.
    Article Snippet: Three-fragment multisite gateway system (Invitrogen, Thermo Fisher Scientific, USA) and In-fusion (Clontech Laboratories, Inc., USA) methods were used to generate most plasmids as previously described34,73,74.

    Article Title: Sensory plasticity caused by up-down regulation encodes the information of short-term learning and memory.
    Article Snippet: All expression plasmids used in this study were constructed by Three-Fragment Multisite gateway system (InvitrogenTM, Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Tissue-Specific Transcription Footprinting Using RNA PoI DamID (RAPID) in Caenorhabditis elegans
    Article Snippet: Reporter plasmids were assembled using the three-fragment MultiSite Gateway system (Thermo Fisher Scientific).

    Article Title: Molecular and circuit mechanisms underlying avoidance of rapid cooling stimuli in C. elegans
    Article Snippet: Three-fragment multisite gateway system (Invitrogen, Thermo Fisher Scientific, USA) and In-fusion (Clontech Laboratories, Inc., USA) methods were used to generate most plasmids as previously described , , .

    Article Title: GCY-20 signaling controls suppression of Caenorhabditis elegans egg laying by moderate cold.
    Article Snippet: All expression constructs were generated with a Three-Fragment Multisite Gateway system (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Dissecting Molecular and Circuit Mechanisms for Inhibition and Delayed Response of ASI Neurons during Nociceptive Stimulus.
    Article Snippet: Briefly, three entry clones containing three PCR products (Promoter, gene of interest, sl2::GFP, or 30UTR, in name of slot1, slot2 and slot3, respectively) were recombined into the pDESTTM R4-R3 Vector II or custom-modified destination vectors using LR recombination reactions to generate the expression plasmids.

    Generated:

    Article Title: The exocytosis regulator complexin controls spontaneous synaptic vesicle release in a CAPS-dependent manner at C. elegans excitatory synapses.
    Article Snippet: All expression plasmids were constructed with a three-fragment multisite gateway system (Invitrogen, Thermo Fisher Scientific, Waltham, Massachusetts, United States of America).

    Article Title: Molecular and circuit mechanisms underlying avoidance of rapid cooling stimuli in C. elegans.
    Article Snippet: Three-fragment multisite gateway system (Invitrogen, Thermo Fisher Scientific, USA) and In-fusion (Clontech Laboratories, Inc., USA) methods were used to generate most plasmids as previously described34,73,74.

    Article Title: Sensory plasticity caused by up-down regulation encodes the information of short-term learning and memory.
    Article Snippet: All expression plasmids used in this study were constructed by Three-Fragment Multisite gateway system (InvitrogenTM, Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Tissue-Specific Transcription Footprinting Using RNA PoI DamID (RAPID) in Caenorhabditis elegans
    Article Snippet: Reporter plasmids were assembled using the three-fragment MultiSite Gateway system (Thermo Fisher Scientific).

    Article Title: Molecular and circuit mechanisms underlying avoidance of rapid cooling stimuli in C. elegans
    Article Snippet: Three-fragment multisite gateway system (Invitrogen, Thermo Fisher Scientific, USA) and In-fusion (Clontech Laboratories, Inc., USA) methods were used to generate most plasmids as previously described , , .

    Article Title: GCY-20 signaling controls suppression of Caenorhabditis elegans egg laying by moderate cold.
    Article Snippet: All expression constructs were generated with a Three-Fragment Multisite Gateway system (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Dissecting Molecular and Circuit Mechanisms for Inhibition and Delayed Response of ASI Neurons during Nociceptive Stimulus.
    Article Snippet: Briefly, three entry clones containing three PCR products (Promoter, gene of interest, sl2::GFP, or 30UTR, in name of slot1, slot2 and slot3, respectively) were recombined into the pDESTTM R4-R3 Vector II or custom-modified destination vectors using LR recombination reactions to generate the expression plasmids.



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